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Expression of histone deacetylase (HDAC)1, 2, 3, and 8 in cultured renal proximal tubular cells (RPTCs). Cultured RPTCs were harvested and cell lysates were subjected to immunoblot analysis with antibodies against HDAC1, 2, 3, or, 8 and GAPDH. Representative immunoblot from 3 or more experiments are shown (A). RPTCs were seeded on coverslips and then cultured with 5% FBS for 24 h. Cells were stained with antibodies against HDAC1, 2, 3, or 8 (×200) and DAPI (B).
