Fig. 2.

Effect of MS-275 on RPTC proliferation. RPTCs were cultured in the DMEM with 5% FBS for 48 h in the absence or presence of MS-275 (0.5–4 μM; A–E). Cells were treated with H₂O₂ (1 mM) for 4 h as positive control of apoptosis (E). Phase-contrast photographs (×200) showing the effect of MS-275 on cell proliferation (A). Cell proliferation was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (B) and cell counting (C). Data are expressed as means ± SE. Means with different superscript letters are represented as significant difference from one another (P < 0.05). Cells were harvested and cell lysates were subjected to immunoblot analysis with antibodies to acetylated histone H3 (acetyl-H3) and α-tubulin (D) or cleaved poly(ADP-ribose)polymerase (PARP), cleaved caspase-3, and α-tubulin (E). Representative immunoblots from 3 or more experiments are shown.