Fig. 4.

Effects of MS-275 or knockdown of HDAC1, 2, 3, or 8 on the expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 in RPTCs. RPTCs were cultured in the DMEM with 5% FBS for 48 h in the absence or presence of MS-275 (0.5 to 4 uM). Cells were harvested and cell lysates were subjected to immunoblot analysis with antibodies against PCNA, cyclin D1, or α-tubulin. Representative immunoblots from 3 or more experiments are shown (A). Expression levels of PCNA and cyclin D1 were calculated by densitometry and normalized with α-tubulin (B). Data are expressed as means ± SE. Means with different superscript letters are represented as significant difference from one another (P < 0.05). RPTCs were transfected with scrambled siRNA or specific siRNA to HDAC1, 2, 3, or 8 and incubated for 48 h in the DMEM with 5% FBS. Prepared cell lysates were subjected to immunoblot analysis with antibodies to PCNA, cyclin D1, or α-tubulin. Representative immunoblots from 3 or more experiments are shown (C–F). Expression levels of PCNA and cyclin D1 were calculated by densitometry and normalized with α-tubulin (G–J). Data are expressed as means ± SE. *P < 0.05, significantly different from controls.