Fig. 2.

Cellular localization of HO-1 expression. A: whole cell lysates and mitochondria were analyzed for HO-1 expression by Western blot analysis. HO-1 is overexpressed in the HO-1 and Mito-HO-1 cell lysates. A higher-molecular-weight protein in the Mito-HO-1 cell lysates represents the unprocessed HO-1. HO-1 is expressed in the mitochondria of only Mito-HO-1 cells. Voltage-dependent anion channel (VDAC, mitochondrial marker) and actin (cytosol marker) serve as loading controls. The absence of actin in the mitochondrial fraction indicates the purity of the mitochondria prep. B and C: mitochondria were labeled with Mitotracker Red (B) or cytochrome c oxidase (C), and cells were immunostained with anti-HO-1 antibody. HO-1 colocalized with mitochondria only in Mito-HO-1 cells.