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. 2013 May 24;305(3):H330–H343. doi: 10.1152/ajpheart.01010.2011

Fig. 6.

Fig. 6.

Effects of siRNA knockdown of thioredoxin reductase-1 (TrxR-1) in BPA on force generation to 25 mM KCl under aerobic and hypoxic conditions and PKG1α dimerization and VASP phosphorylation under hypoxia. A: Western blot analysis showing that TrxR-1 siRNA transfection for 48 h decreased expression of TrxR-1 (blot on right) compared with scrambled siRNA controls (blot on left from the same Western blot and animal) (n = 7). B: with TrxR-1 siRNA transfection, contraction to 25 mM potassium under aerobic and hypoxic conditions is attenuated (n = 7) compared with scrambled siRNA controls. C and D: Western blot analysis showing that TrxR-1 siRNA transfection for 48 h increased the observed level of PKG1α dimerization (n = 8; C) and VASP phosphorylation (n = 8; D) under hypoxia compared with scrambled siRNA controls. *P < 0.05 vs. scrambled siRNA control response; #P < 0.05 vs. scrambled siRNA control dimerization response.