TABLE 1.
C. parasitica genes responsive to infection by both CHV1-EP713 and CHV1-Euro7a
| Category | 713vs155
|
AEST ID | Euro7vs155
|
E-value | Description (organism) | ||
|---|---|---|---|---|---|---|---|
| Direction | Fold change | Direction | Fold change | ||||
| Amino acid metabolism | UP | 23.69 | [13] AEST-08-F-10 | UP | 5.80 | 5.00E-95 | SAMS (Neurospora crassa) |
| DN | 4.95 | AEST-22-H-07 | DN | 2.24 | 1.00E-20 | Putative tyrosinase (Gibberella zeae) | |
| Carbohydrate metabolism | DN | 3.43 | AEST-19-G-03 | DN | 2.22 | 1.00E-74 | Hypothetical protein (Neurospora crassa) |
| 5.00E-71 | 1,2-α-Mannosidase (Aspergillus phoenicis) | ||||||
| DN | 4.28 | AEST-30-G-01 | DN | 1.76 | 3.00E-56 | Hypothetical protein (Neurospora crassa) | |
| 9.00E-44 | Acid-stable alpha-amylase (Aspergillus kawachii) | ||||||
| DN | 4.92 | AEST-37-F-03 | DN | 2.13 | 1.00E-52 | β-Glucosidase homolog (Cochliobolus heterostrophus) | |
| Cell wall or growth | DN | 5.53 | AEST-20-G-02 | DN | 1.94 | 2.00E-27 | Predicted protein (Neurospora crassa) |
| 4.00E-10 | Hard surface-induced protein 3 (Glomerella cingulata) | ||||||
| Development | DN | 3.94 | AEST-30-F-01 | DN | 1.95 | 6.00E-23 | Clock-controlled protein 6 (Neurospora crassa) |
| Electron transport | UP | 2.11 | AEST-33-E-05 | DN | 2.46 | 2.00E-32 | Hypothetical protein (Neurospora crassa) |
| 1.00E-17 | Oxidoreductase, MmyG [Streptomyces coelicolor A3(2)] | ||||||
| UP | 8.14 | AEST-33-G-12 | UP | 3.67 | 2.00E-17 | Hypothetical protein (Burkholderia fungorum) | |
| 1.00E-15 | Oxidoreductase (Pseudomonas syringae) | ||||||
| UP | 3.20 | AEST-35-E-02 | DN | 2.86 | 9.00E-23 | Hypothetical protein (Microbulbifer degradans 2-40) | |
| 4.00E-19 | Oxidoreductase (Clostridium acetobutylicum) | ||||||
| Lipid metabolism | UP | 3.69 | AEST-11-C-02 | UP | 3.81 | 7.00E-84 | Hypothetical protein (Neurospora crassa) |
| 1.00E-61 | Similar to LTA4 hydrolase (Homo sapiens) | ||||||
| Metabolism related | DN | 6.19 | [11] AEST-06-B-03 | DN | 3.16 | 2.00E-32 | Unnamed protein product (Podospora anserina) |
| 3.00E-30 | Possible CGI-83 protein (Leishmania major) | ||||||
| UP | 5.36 | [26] AEST-17-H-10 | DN | 2.02 | 3.00E-32 | Isoamyl alcohol oxidase (Neurospora crassa) | |
| UP | 18.83 | AEST-20-A-03 | UP | 5.23 | 3.00E-57 | FK506-binding protein (Neurospora crassa) | |
| UP | 3.99 | AEST-28-C-01 | UP | 2.03 | 3.00E-20 | Hypothetical protein (Neurospora crassa) | |
| 4.00E-12 | Putative acetyltransferase (Clostridium tetani E88) | ||||||
| UP | 3.11 | AEST-28-D-08 | UP | 2.02 | 4.00E-22 | Short-chain dehydrogenase (Schizosaccharomyces pombe) | |
| DN | 1.79 | AEST-36-F-03 | DN | 1.88 | 2.00E-22 | Hypothetical protein (Neurospora crassa) | |
| 3.00E-13 | Aflatoxin B1 aldehyde reductase 1 (Mus musculus) | ||||||
| Nucleic acid metabolism | DN | 6.20 | [9] AEST-05-C-02 | DN | 2.31 | 3.00E-27 | Hydrogenase regulation HoxX (Aquifex aeolicus) |
| UP | 11.45 | AEST-08-E-11 | UP | 5.13 | 5.00E-47 | Hypothetical protein (Neurospora crassa) | |
| 8.00E-19 | ATP synthase/rho (Brucella melitensis) | ||||||
| UP | 3.11 | [17] AEST-11-E-06 | UP | 2.91 | 4.00E-29 | Hypothetical protein (Neurospora crassa) | |
| 7.00E-22 | Transcriptional regulator (Clostridium tetani E88) | ||||||
| UP | 2.02 | [31] AEST-24-D-01 | UP | 2.92 | 2.00E-32 | SnodProt1 precursor (Neurospora crassa) | |
| UP | 11.04 | AEST-28-F-06 | UP | 2.00 | 7.00E-48 | Hypothetical protein (Neurospora crassa) | |
| 2.00E-14 | Probable AAA family ATPase (Campylobacter jejuni) | ||||||
| Oxygen/radical metabolism | UP | 8.90 | [38] AEST-31-F-09 | UP | 4.67 | 3.00E-39 | Hypothetical protein (Neurospora crassa) |
| 5.00E-35 | Fum12p (Gibberella moniliformis) | ||||||
| Protein metabolism | DN | 4.73 | [8] AEST-05-A-09 | DN | 2.08 | 2.00E-53 | Aspergillopepsin II precursor (Aspergillus niger) |
| UP | 9.86 | AEST-05-E-01 | UP | 5.44 | 2.00E-66 | Probable zinc metallo-protease (Neurospora crassa) | |
| DN | 3.19 | AEST-08-C-09 | DN | 2.19 | 5.00E-92 | Acid proteinase (Cryphonectria parasitica) | |
| DN | 3.91 | AEST-11-D-08 | DN | 2.63 | 1.00E-109 | Endothiapepsin precursor (Cryphonectria parasitica) | |
| DN | 2.99 | [24] AEST-17-C-03 | UP | 2.15 | 1.00E-33 | Aspartic protease precursor (Botryotinia fuckeliana) | |
| DN | 3.09 | AEST-18-C-11 | DN | 2.01 | 4.00E-72 | Carboxypeptidase S1 (Penicillium janthinellum) | |
| UP | 5.51 | AEST-38-C-10 | UP | 2.48 | 5.00E-54 | Ubiquitin-conjugating enzyme E2 (Magnaporthe grisea) | |
| Stress response | UP | 5.61 | AEST-10-H-10 | UP | 2.73 | 2.00E-77 | Heat shock 70-kDa protein (Ajellomyces capsulatus) |
| UP | 5.39 | [21] AEST-12-G-04 | DN | 4.04 | 3.00E-67 | Hypothetical protein (Neurospora crassa) | |
| 2.00E-38 | GST (Schizosaccharomyces pombe) | ||||||
| DN | 4.00 | AEST-38-C-04 | DN | 3.91 | 1.00E-108 | HSP70 (Neurospora crassa) | |
| Transport | UP | 9.31 | AEST-01-G-12 | UP | 3.24 | 4.00E-61 | Hypothetical protein (Neurospora crassa) |
| 1.00E-40 | Nontransporter ABC protein (Dictyostelium discoideum) | ||||||
| UP | 3.76 | [27] AEST-22-A-11 | UP | 1.90 | 2.00E-68 | Stomatin-like protein (Gibberella fujikuroi) | |
| DN | 3.44 | AEST-27-E-05 | DN | 3.03 | 3.00E-25 | Acetylcholine transporter (Schizosaccharomyces pombe) | |
| Unknown | UP | 5.05 | AEST-01-B-11 | UP | 2.51 | ||
| UP | 8.78 | AEST-01-C-10 | UP | 4.20 | 4.00E-05 | Predicted protein (Neurospora crassa) | |
| UP | 3.96 | AEST-01-E-12 | UP | 2.45 | 1.00E-36 | Unnamed protein product (Podospora anserina) | |
| UP | 4.55 | AEST-02-F-06 | DN | 1.91 | 3.00E-12 | Hypothetical protein (Rhodopseudomonas palustris)/PICK> | |
| DN | 8.98 | [7] AEST-04-D-04 | DN | 5.12 | |||
| DN | 6.78 | [10] AEST-05-D-11 | DN | 5.60 | |||
| DN | 3.90 | AEST-07-A-11 | DN | 2.01 | |||
| UP | 7.09 | AEST-09-B-02 | UP | 2.04 | 1.00E-04 | Hypothetical protein (Neurospora crassa) | |
| UP | 6.19 | AEST-09-G-04 | UP | 3.62 | 2.00E-04 | Glu-Asp-rich protein (Dictyostelium discoideum) | |
| DN | 5.73 | AEST-09-G-11 | DN | 2.74 | |||
| UP | 7.89 | AEST-09-H-05 | UP | 3.94 | |||
| UP | 6.22 | AEST-09-H-07 | UP | 3.66 | 1.00E-05 | Hypothetical protein (Neurospora crassa) | |
| DN | 5.39 | AEST-11-A-09 | DN | 2.30 | 2.00E-05 | Probable aldolase (Nostoc sp. strain PCC 7120) | |
| DN | 9.68 | AEST-12-C-05 | DN | 2.04 | 1.00E-09 | Membrane glycoprotein (Equine herpesvirus 1) | |
| UP | 7.88 | AEST-13-E-03 | UP | 3.55 | 9.00E-30 | Predicted protein (Neurospora crassa) | |
| DN | 3.11 | AEST-15-C-06 | DN | 2.41 | 3.00E-36 | Predicted protein (Neurospora crassa) | |
| UP | 2.38 | AEST-15-F-06 | UP | 2.08 | 3.00E-27 | Predicted protein (Neurospora crassa) | |
| DN | 3.30 | AEST-16-A-02 | DN | 1.94 | 8.00E-05 | Hypothetical protein (Neurospora crassa) | |
| DN | 5.44 | AEST-16-A-07 | DN | 2.32 | 8.00E-03 | Cholesterol oxidase precursor (Neurospora crassa) | |
| DN | 4.58 | AEST-16-A-09 | DN | 1.77 | 3.00E-17 | Predicted protein (Neurospora crassa) | |
| UP | 3.79 | AEST-16-C-12 | UP | 2.34 | 4.00E-03 | Hypothetical protein L1177.03 (Leishmania major) | |
| UP | 5.81 | AEST-17-G-12 | UP | 3.21 | 1.00E-06 | Predicted protein (Neurospora crassa) | |
| UP | 15.68 | AEST-18-C-02 | UP | 4.66 | |||
| UP | 6.56 | AEST-18-C-10 | UP | 2.01 | |||
| UP | 5.51 | AEST-19-A-08 | UP | 3.59 | 2.00E-27 | Predicted protein (Neurospora crassa) | |
| DN | 5.76 | AEST-19-A-11 | DN | 2.18 | |||
| DN | 3.05 | AEST-19-C-02 | DN | 1.77 | 1.00E-07 | Polyketide synthase (Cochliobolus heterostrophus) | |
| UP | 5.54 | AEST-19-G-01 | UP | 3.29 | 2.00E-04 | Class A calcium channel variant (Rattus norvegicus) | |
| DN | 3.77 | AEST-19-H-08 | DN | 2.19 | |||
| DN | 3.22 | AEST-20-C-06 | DN | 1.79 | 2.00E-10 | Acid phosphatase precursor (Yarrowia lipolytica) | |
| DN | 5.87 | AEST-20-D-11 | DN | 3.26 | 5.00E-03 | Predicted protein (Neurospora crassa) | |
| DN | 3.29 | AEST-20-E-08 | DN | 2.21 | |||
| DN | 11.26 | AEST-21-A-03 | DN | 2.08 | 2.00E-08 | Hypothetical protein XP_211670 (Homo sapiens) | |
| DN | 3.88 | AEST-21-D-04 | DN | 2.26 | 4.00E-09 | Hypothetical protein (Neurospora crassa) | |
| DN | 10.79 | AEST-21-E-08 | DN | 2.39 | 8.00E-07 | YFW1 gene (Saccharomyces cerevisiae) | |
| UP | 4.92 | AEST-21-E-10 | UP | 2.14 | 2.00E-24 | Predicted protein (Neurospora crassa) | |
| UP | 4.61 | AEST-21-H-05 | UP | 2.50 | 9.00E-05 | Proteophosphoglycan (Leishmania major) | |
| UP | 3.08 | AEST-22-G-02 | UP | 1.81 | 1.00E-07 | Hypothetical protein XP_209582 (Homo sapiens) | |
| DN | 4.75 | AEST-23-B-10 | DN | 2.67 | 2.00E-05 | Keratin-associated protein 4.12 (Mus musculus) | |
| UP | 3.94 | [33] AEST-26-B-03 | DN | 1.81 | 1.00E-36 | Predicted protein (Neurospora crassa) | |
| UP | 6.06 | AEST-27-E-11 | UP | 4.29 | |||
| UP | 10.48 | AEST-30-B-02 | UP | 3.72 | |||
| DN | 4.38 | AEST-36-C-10 | DN | 3.70 | |||
| DN | 3.06 | AEST-39-H-07 | DN | 2.04 | 2.00E-06 | Glycoprotein gp2 precursor (Caenorhabditis elegans) | |
a
The AEST library identification (AEST ID [1]) for each gene is indicated in column 4. Clones for which real-time RT-PCR confirmations were performed are preceded by a number in brackets that refers to the corresponding clone in Table 3. Two clone IDs, [17] AEST-11-E-06 and [33] AEST-26-B-03, indicate a discrepancy between the microarray and real-time RT-PCR results. The direction and magnitude (average fold change for six hybridizations) of change in transcript accumulation for each gene after CHV1-EP713 infection are indicated in columns 2 and 3, respectively. Similarly, the direction and magnitude of change in transcript accumulation for each gene after CHV1-Euro7 infection are indicated in columns 5 and 6, respectively. As for column 3, the values in column 6 represent the average fold change for six hybridizations. Column 7 indicates the strength of the BLAST hit corresponding to the biological process description and source organism of the matched sequence in column 8. In cases in which a clone was grouped by using a secondary BLAST hit, the strongest biological process description is listed first, followed by the biological process description used to group the clone. In addition, a few clone IDs listed under the “unknown” ontological category returned no BLAST information and are left blank. Direction: UP, up; DN, down.