Table 1.
DNA sequence analysis of junctions formed between pBR322 and the end of BIR-extended DNA adjacent to LEU2
| Clone | Sequence | Nucleotide at junction | |
|---|---|---|---|
| G423 | pBR322 | ACATTAACCTAT | 4313 (−46) |
| G466 | Junction | ACATTATCCGGT | |
| G574 | Chromosome III | TTACTATCCGGT | 92912 (+1328) |
| G578 | pBR322 | GAGGCCCTTTCG | 4347 (−12) |
| Junction | GAGGCCAAGTCT | ||
| Chromosome III | AAAGACAAGTCT | 95241 (+3657) | |
| G575 | pBR322 | AGGCCCTTTCGT | 4348 (−11) |
| Junction | AGGCCAAGTCTC | ||
| Chromosome III | AAGACAAGTCTC | 95571 (+3987) | |
| G576 | pBR322 | CACGAGGCCCTT | 4344 (−15) |
| Junction | CACGAGTAGCAT | ||
| Chromosome III | TAGGAGTAGCAT | 96632 (+5048) |
Nonhomologous junctions formed by replication-extension of the “LEU” segment of LEU2 and pBR322, illustrated in Fig. 2B. Initially, the ends of EcoRI-linearized pWL37 corresponded to position 4359 in the pBR322 sequence and the EcoRI site of the cloned “LEU” region, corresponding to nucleotide 91584 in the chromosome III sequence (78). The number of nucleotides lost (indicated by a minus sign) or gained (plus sign) from the two EcoRI ends is also shown.