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. 2013 Aug 14;33(33):13569–13580. doi: 10.1523/JNEUROSCI.1197-13.2013

Figure 5.

Figure 5.

SARM forms SAM-mediated complexes that require multiple TIR domains to promote axon degeneration. A, Coimmunoprecipitation of full-length Flag-tagged SARM (SARM-F) with Venus-tagged SARM and deletion mutants. Full-length SARM-V and mutants containing SAM domains (ΔTIR, SAM) interact strongly with full-length SARM (top, Flag blot), whereas little interaction is observed for mutants lacking SAM domains (ΔSAM, TIR). B, Axons of wild-type DRG neurons degenerate within 24 h postaxotomy. Degeneration is unaffected by expression of SARM-V, ΔSAM, ΔSAMΔTIR, or TIR, but is blocked by SARM mutants that lack a TIR domain but contain intact SAM domains (ΔTIR, SAM). C, Representative images of α-tubulin-stained axons from experiment shown in B. D, Time course measurement of axon degeneration following axotomy. While SARM-V expression does not affect the rate of axon degeneration, expression of SARM lacking a TIR domain (ΔTIR) completely blocks axon degeneration for ≥2 d. ***p < 0.001; error bars = SEM, scale bar, 50 μm.