Table 1.
Summary of findings for SARM domain deletion mutants
| Mutant | Residues | SARM−/− rescue | Dominant negative | Toxicity | Localization | SARM binding |
|---|---|---|---|---|---|---|
| SARM-V | 1–724 | + | − | − | Mitochondria | + |
| ΔN27 | 28–704 | + | − | − | Cytosol | NE |
| ΔSAM | 1–409; 561–724 | − | − | − | Mitochondria | − |
| SAM | 409–560 | − | + | − | Cytosol | + |
| ΔTIR | 1–560 | − | + | − | Mitochondria | + |
| TIR | 561–724 | − | − | − | Cytosol | − |
| ΔSAMΔTIR | 1–408 | − | − | − | Mitochondria | NE |
| SAM/TIR | 409–724 | NEa | NEa | + | Cytosol | NEa |
| SAM/TIR [697-704A] | 409–724 | NE | NE | − | Cytosol | NE |
NE, Not evaluated.
aNot evaluated due to toxicity.