Fig. 2.

Schematic of protocol 2. Intact airways were constricted twice in random order. A: Pre + Post: intact airways were exposed to large Ptm oscillations (5–20 cmH₂O, 0.2 Hz) for 30 min before addition of ACh (10⁻⁵ M) and throughout the duration (20 min) of the constriction. B: Post Only: intact airways were constricted with the same dose of ACh under a static Ptm for 5 min (Static loading condition), and then large Ptm oscillations (5–20 cmH₂O) were applied for the next 15 min. Arrows indicate the time at which ACh was added to the bath.