FIG. 1.

Expression of dominant-negative p53 and its effect on HPV E6 and E7 gene expression. At 2 days after infection with the E2 virus (+) or mock infection (−), protein and RNA were harvested from HeLa/LXSN-1, HeLa/LXSN-p53CTF-3, and HeLa/16E7-3 cells, as well as from two HeLa/16E7-p53CTF cell lines, as indicated. In the top panel, 5 μg of protein of each sample was resolved by gel electrophoresis, transferred to a membrane, and probed with antibodies specific for p53CTF. In the bottom panel, 5 μg of RNA of each sample was resolved by gel electrophoresis, transferred to a membrane, and hybridized to a probe specific for the HPV18 E6/E7 region.