FIG. 2.

Effect of dominant-negative p53 expression on the p53 and Rb pathways. At 2 days after infection with the E2 virus (+) or mock infection (−), protein was harvested from HeLa/LXSN-1, HeLa/LXSN-p53CTF-3, HeLa/16E7-3, and HeLa/16E7-RVY-3 cells, as well as from two HeLa/16E7-p53CTF cell lines, as indicated. 5 μg or 10 μg (for cyclin A) of each sample was resolved by gel electrophoresis, transferred to a membrane, and probed with antibodies specific for wild-type p53, p21, p105^Rb, or cyclin A, as indicated. The hyperphosphorylated and hypophosphorylated forms of p105^Rb are indicated by P and O, respectively.