FIG. 8.
Effect of hTERT expression on the p53 and Rb pathways. At 2 days after infection with the E2 virus (+) or mock infection (−), protein was harvested from HeLa/LXSN-1, HeLa/16E7-3, and HeLa/16E7-RVY-3 cells, as well as from two HeLa/16E7-TERT cell lines, as indicated. Then, 5 μg of each sample was resolved by gel electrophoresis, transferred to a membrane, and probed with antibodies specific for wild-type p53, p21, p105Rb, or cyclin A, as indicated. The hyperphosphorylated and hypophosphorylated forms of p105Rb are indicated by P and O, respectively.