Table 1.

Scheme of the experiment. Two spring B. napus L. genotypes: cv ‘DH4079’ line and cv ‘Campino’ were grown under greenhouse conditions at 18/18 °C (day/night) temperature until the beginning of bolting. Then plants were divided into two groups: (1) continuing growth at 18/18 °C (day/night) temperature and (2) transferred to continuous mild temperature of 10/10 °C (day/night). After next 10 days, flower buds were excised from donor plants. Some of excised flower buds (2.6–2.8 mm in length) were collected in Eppendorf tubes and frozen in liquid nitrogen. Some of excised flower buds were used for microspores (mcs) isolation. Isolated mcs were cultured at a density of 40,000 ml⁻¹ in NLN-13 culture medium. Mcs were cultured at various culture temperature regimes: (1) 18 °C, and (2) 32 ± 0.2 °C for 24 h and then 25 °C

Genotypes of Brassica napus L. in tissue/cell	Growth conditions of donor plants	Type of tissue/cells	Measurements
			Membrane fluidity	ABA content or concentration in tissue/cell	ABA content in NLN-13 medium	pH value in culture medium
‘DH4079’ line/‘Campino’	18/18 °C (day/night)	Flower buds		X
		mcs on the isolation day		X
		mcs 1 day onNLN-13 medium at 18 °C		X	X	X
		mcs 1 day on NLN-13 medium at 32 °C		X	X	X
	10/10 °C (day/night)	Flower buds		X
		mcs on the isolation day		X
		mcs1lday onNLN-13 medium at 18 °C	X	X	X	X
		mcs 1 day on NLN 13 medium at 32 °C	X	X	X	X