Fig. 9.

Total mercury content and cathepsin L activity in the exosomal fraction following MeHg exposure. Secreted exosomes were collected from the culture medium by ultracentrifugation at 100,000×g for 1 h. a Total mercury content in the exosomal fraction. b Activity levels of the lysosomal marker enzyme cathepsin L in the exosomal fraction. To inhibit lysosomal ATPase function, 5 nM baf was added to the culture medium at 12 hpf. Values and bars indicate means ± SE of three independent experiments. OCTN1-deficient embryos (antisense MO1) and control embryos (5′-mis control MO1) were compared. Asterisks indicate significant differences (ANOVA; P < 0.05)