Changes of NO level in hippocampus slices and HCN1 expression in CA1 region during Hb perfusion. A, Concentration of NO released from CA1 slices at 1, 2, 3, and 4 h after Hb perfusion. n = 5 rats per group. B, Western blot for HCN1 in CA1 slices at 1, 2, 3, and 4 h after Hb perfusion. The expression levels are expressed as a ratio of β-actin levels for normalization. n = 5 rats per group.