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. 2012 Feb 29;32(9):3164–3175. doi: 10.1523/JNEUROSCI.5143-11.2012

Figure 9.

Figure 9.

Changes of HCN1 protein and mRNA expression in hippocampus CA1 region after SAH. A, Schematic illustration of sectioning of hippocampus technique for regional tissue acquisition and analysis. B, Western blot for HCN1 in the right hippocampus CA1 region at 24 and 72 h after SAH. The expression levels are expressed as a ratio of β-actin levels for normalization. n = 5 rats per group. C, Top, Reverse transcription-PCR assay of HCN1 mRNA expression in the right hippocampus CA1 region at 24 and 72 h after SAH. Bottom, Real-time quantitative PCR measurements of HCN1 mRNA expression in the right hippocampus CA1 region at 24 and 72 h after SAH. mRNA expressed as a percentage change from control. n = 6 rats per group.