TABLE 1.

NP-specific ELISPOT data for vaccinated mice pre- and postchallenge^a

T-cell type, vaccine, and cytokine	Data for spleen				Data for lungs
	Pre	Post 1	Post 2	Avg increase (n-fold)	Pre	Post 1	Post 2
CD4
sHA-NP
IL-4	166 ± 12	3,744 ± 158	3,307 ± 370	21	<b	NDc	316 ± 78
IFN-γ	76 ± 7	1,238 ± 121	1,096 ± 155	15	<	ND	1,213 ± 236
tmHA-NP
IL-4	181 ± 12	3,540 ± 157	2,902 ± 317	18	<	ND	341 ± 250
IFN-γ	97 ± 4	1,200 ± 101	2,300 ± 259	18	<	ND	1,296 ± 259
CD8
sHA-NP
IL-4	179 ± 11	641 ± 54	882 ± 276	4	<	<	<
IFN-γ	85 ± 2	2,100 ± 141	3,624 ± 701	34	<	6,321 ± 210	9,016 ± 1,125
tmHA-NP
IL-4	116 ± 5	861 ± 23	781 ± 237	7	<	<	<
IFN-γ	237 ± 11	4,294 ± 153	4,355 ± 552	18	<	15,124 ± 226	12,487 ± 2,243

^aValues represent the average numbers of NP-specific T cells producing the indicated cytokine (per million white blood cells) as detected by ELISPOT for three mice per group. Pre is prechallenge data, and Post 1 and Post 2 are postchallenge data for two subsets of a group of mice that were immunized at the same time but challenged at different times. Postchallenge, the total number of cells recovered from the lungs increased approximately fourfold, whereas no increase in the total number of recovered cells occurred for spleens.

^b<, below detection threshold.

^cND, not done.