Table 2.
Mutation assayed | Diploid strain | Growth temperature, °C | Results of tetrad analysis
|
Nature of genetic interaction with rad27Δ | |||
---|---|---|---|---|---|---|---|
Number of viable spores per tetrad (number of tetrads)
| |||||||
4 | 3 | 2 | 1+0 | ||||
rad52-Δ251 | ORD 5946 | 30 | 3 | 9 | 3 | 2 | Colethal |
rad52-Δ327 | ORD 5947 | 30 | 6 | 17 | 13 | 0 | Colethal |
mre11-D16A | ORD 5936 | 30 | 5 | 25 | 9 | 0 | Colethal |
mre11-6 | ORD 5916 | 30 | 4 | 32 | 11 | 2 | Colethal |
mre11-58S | ORD 5918 | 30 | 3 | 11 | 6 | 0 | Colethal |
mre11-5 | ORD 5917 | 30 | 16 | 0 | 4 | 0 | Viable |
mre11ΔC49 | ORD 5938 | 30 | 25 | 13 | 2 | 0 | Viable |
rfa1-t11 | ORD 5310 | 30 | 4 | 21 | 10 | 0 | Colethal |
ORD 5310 | 23 | 3 | 16 | 5 | 0 | Colethal | |
rfa1-t48 | ORD 5319 | 30 | 0/32 rad27Δ colonies were double-mutant | Colethal | |||
ORD 5319 | 23 | 0/23 rad27Δ colonies were double-mutant | Colethal |
Results of sporulation of diploid in which the rad27 deletion and the x mutation could not be individually distinguished (see Materials and Methods). The data indicate the number of viable spores per tetrad.