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. 2013 Jun 6;305(3):L267–L277. doi: 10.1152/ajplung.00288.2012

Fig. 5.

Fig. 5.

Sulforaphane restored Nrf2/ARE activity in L2 cells treated with the HIV-1-related protein gp120 and increased Nrf2 and NQO-1 protein expression as well as the GSH-to-GSSG ratios in primary AEC from HIV-1 Tg rats. A: sulforaphane treatment (1 μM) restored the relative Nrf2/ARE activity (by a dual-luciferase reporter assay) in L2 cells treated with gp120 (n = 11). B: representative Western blot and summary of densitometry data (n = 6) showed relative Nrf2 and NQO1 protein expression in primary AEC from HIV-1 Tg rats untreated or treated with sulforaphane (1 μM) for 4 days. C: relative intracellular GSH-to-GSSG ratios were measured in primary AEC from WT and HIV-1 Tg rats cultured for 4 days ± sulforaphane (1 μM) (n = 6). *P < 0.05 compared with untreated L2 cells (A) or cells from WT rats (C). #P < 0.05 compared with L2 cells treated with gp120 (A) or with cells from HIV Tg rats (C).