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Relative quantity of moesin (MSN), ezrin (EZR), and radixin (RDX) (i.e., ERM) mRNA in human pulmonary artery endothelial cells (HPAEC). Total RNA was isolated from HPAEC and quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed by using target gene specific primers and probes and the relative amounts were expressed by standard curve method as described in materials and methods. Each value represents the mean of triplicates.
