Fig. 11.

Distribution of phospho-ERM in EC after thrombin. EC grown on glass coverslips and treated with 0.5 U/ml thrombin for indicated time (B–E) or nontreated control cells (A) were subjected to immunofluorescent staining with anti-phospho-ERM Ab. The phospho-ERM signal is very weak in quiescent monolayers and is evident only in spikelike structures in cell-cell border areas (A, arrow 1). Threonine-phosphorylated ERM proteins predominantly localized to the periphery of ECs following thrombin stimulation (5–15 min, B and C, arrow 2) and also are detectable in peripheral spikelike structures (B, arrow 1). After 1–2 h phosphorylated ERM localized in spikelike structures characteristic of quiescent cells and in cytoplasm (D and E). Images are representative of 3 independent experiments. Scale bar = 10 μm.