Fig. 2.

Time-dependent effects of thrombin on threonine phosphorylation of ERM. A: confluent HPAEC were treated either with control vehicle or thrombin (0.5 U/ml) for the indicated times, and phosphorylated ERM [phospho-ezrin (Thr567)/radixin (Thr564)/moesin (Thr558)] was detected via immunoblotting. B: bar graph represents relative densitometry. Data are presented as fold changes in phosphorylated ERM over vehicle-treated control and expressed as means ± SE from 3 independent experiments. *P < 0.05 vs. unstimulated control.