Fig. 9.

Effects of PAR₁ blocking antibodies on thrombin-induced ERM phosphorylation. A: HPAEC were pretreated for 1 h with either control vehicle or the PAR₁ blocking Abs ATAP2 (25 μg/ml) or WEDE15 (25 μg/ml) or with combination of ATAP2 and WEDE15, then stimulated by thrombin (0.5 U/ml, 5 min) or vehicle. Total lysates were analyzed by immunoblotting for phospho-ERM. Immunoblotting with β-actin Ab was used as a normalization control. B: bar graph represents relative densitometry of fold changes in phosphorylated ERM after thrombin relative to vehicle-treated control. Results are means ± SE of 4 independent experiments. *Significantly different from cells treated with ns siRNA without sphingosine-1 phosphate (S1P) (P < 0.05); #significantly different from cells treated with ns siRNA without thrombin (P < 0.01). **Significantly different from cells treated with ns siRNA and thrombin (P < 0.05).