Activation of Wnt3a and Wnt5a during transdifferentiation. Representative immunoblots of Wnt3a, Wnt5a, and prosurfactant protein-C (pro-SPC) from lysate from ATII cells cultured from 0 to 6 days are shown (A, B, and C, respectively). β-Actin was immunoblotted as a loading control. D: densitometry for Wnt3a, Wnt5a, and Pro-SPC (n = 4). *Significant difference from day 0 (P < 0.05). E and F: representative immunoblots and densitometry of Wnt5a (n = 5) and pro-SPC (n = 3) in A549 cells treated with IGF-I for 72 h. *Significant difference from untreated control (P < 0.05).