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. 2013 May 24;305(3):L222–L228. doi: 10.1152/ajplung.00014.2013

Fig. 6.

Fig. 6.

Knockdown of Wnt5a by small-interfering RNA (siRNA) increased the expression of pro-SPC. Wnt5a was knocked down by siRNA in ATII cells according to the procedure described in materials and methods. A: immunoblot of Wnt5a from lysates from 4 different isolations with corresponding densitometry. β-Actin was used as a loading control. *Significant difference from cells transfected with control siRNA (P < 0.05). B: expression of pro-SPC was examined by immunofluorescence in control and Wnt5a siRNA-transfected cells. Mean fluorescence intensity was measured in 5 different fields from 5 different experiments. *Significant difference from cells transfected with control siRNA (P < 0.05; n = 5); NS, values were not significantly different from control siRNA; #significant difference from cells transfected with Wnt5a siRNA.