Fig. 7.

Wounding stimulated Wnt5a expression, and IGF-I and Wnt5a augmented wound healing in MLE-12 cells. A: MLE-12 cells were either unwounded (control) or wounded with multiple scratches, and Wnt5a expression was determined from cell lysates after 36 h. A representative immunoblot is shown, and densitometry is summarized. *Significant difference from unwounded control (P < 0.05, n = 3). B: MLE-12 cells were wounded and then treated with either murine IGF-I (50 ng/ml), murine recombinant Wnt5a (100 ng/ml), or the combination of the two. IGF-I and Wnt5a were added at time 0 and at 24 h, and wound width measurements were made at the initial time and after 36 h. *Significant difference compared with control (P < 0.05; n = 6).