FIG. 5.
Kinetics of Erk phosphorylation by Ad7 in bovine and human lung slice models. Normal bovine (A to C) or human (D to F) lung slices were stimulated with either 2 × 109 PFU of Ad7/ml, virus-free buffer (Mock) or 100 ng of PMA/ml for various times. Tissue lysates were analyzed for Erk activation by immunoblotting for both phosphorylated (active) and total Erk. Panels A and D show Western blots performed with antibody specific for phosphorylated Erk 1/2. Panels B and E are the same blots stripped and reprobed with an antibody specific for both phosphorylated and nonphosphorylated forms of Erk 1/2. Panels C and F show Erk activation graphed as the ratio of active, phosphorylated Erk to total Erk.