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. 2013 Apr 17;33(16):6950–6963. doi: 10.1523/JNEUROSCI.0277-13.2013

Figure 4.

Figure 4.

Calcineurin inhibition with FK506 totally blocks AIS relocation in all excitatory cell types. A, FK506 at 1 μm totally inhibits DGC AIS relocation. Left, Example images of DGCs treated with DMSO or 1 μm FK506 in NaCl or KCl treatment groups. Right, Mean ± SEM of AIS start (S), maximum (M), and end (E) for each treatment and subsequent calculation of AMI. B, FK506 inhibits AIS relocation even at low concentrations. Left, Example images of DGCs depolarized for 48 h in the presence of either 100 or 10 nm FK506. Right, AMI mean ± SEM values for DGCs after FK506 treatment. C, FK506 at 1 μm blocks AIS relocation in other excitatory cell types. Left, Example images of CA1 and CA3 neurons depolarized for 48 h in the presence of 1 μm FK506. Right, AMI mean ± SEM values for CA1 and CA3 neurons after FK506 treatment. ***p < 0.0001, single-sample t test of AMI vs 1. Numbers within bars show the number of cells for each experiment. Scale bars, 10 μm. Thick white line denotes axon start, and white triangles illustrate AIS location. AnkG, Ankyrin-G; Px1, prox1.