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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Hypertension. 2012 Sep 4;60(4):981–990. doi: 10.1161/HYPERTENSIONAHA.112.201509

Figure 6. Phosphorylation of SPAK and OSR1 by WNK kinases is required for the high blood pressure and increased NCC phosphorylation of db/db mice.

Figure 6

A. Systolic blood pressure of SpakT243A/+ and Osr1T185A/+ knock-in db/db mice. Increased systolic blood pressure of db/db mice corrected in SPAKT243A/+ or OSR1T185A/+ knock-in mice (threonines in T-loop mutated to alanine to prevent activation by WNK kinases). Mean ± SEM. (n as indicated). *p<0.05.

B. Representative immunoblot of renal NCC phosphorylation in SPAKT243A/+ and OSR1T185A/+ knock-in db/db mice. Compared to db/db controls, NCC phosphorylation was decreased.

C. Densitometry analyses of renal NCC phosphorylation of NCC in SPAKT243A/+ and OSR1T185A/+ knock-in db/db mice. Values expressed as the ratio to the average of signals in vehicle group. Mean ± SEM. (n as indicated). *p<0.05.