FIG. 3.

Effect of HSV-1 on the accumulation of Stat1 and Stat2 protein levels. Replicate HeLa cell cultures were either mock infected or exposed to 10 PFU of HSV-1(F) per cell and then harvested at 2, 4, 8, 12, or 16 h after infection, processed, subjected to electrophoresis in denaturing gels (200 μg of protein per lane), transferred to a nitrocellulose sheet, and reacted with anti-Stat1 (A) or Stat2 (B) antibodies and then with secondary antibodies conjugated to peroxidase. Bound antibodies were detected by using enhanced chemiluminescence and quantified with the aid of the Storm 860 PhosphorImager. The results were normalized to the Stat protein levels in the uninfected cell samples.