Skip to main content
. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: J Endocrinol. 2009 Apr 16;202(1):77–86. doi: 10.1677/JOE-09-0119

Figure 5.

Figure 5

Thapsigargin Had Little or No Effect Alone or on AngII-Stimulated Aldosterone Secretion but Enhanced Steroidogenesis in Response to Elevated [K+]e and PMA in Primary Cultures of Bovine Adrenal Glomerulosa Cells. (A) Bovine adrenal glomerulosa cells were incubated for 1 hour with a bicarbonate-buffered Kreb’s Ringer solution with 2.5 mM sodium acetate (KRB+) containing no additions (Con), 15 mM KCl (K+; iso-osmotic substitution with Na+) or 10 nM AngII in the presence or absence of 2 μM thapsigargin (Thaps) or vehicle (0.1-0.2% DMSO). Supernatants were collected and assayed for aldosterone secretion by radioimmunoassay. Values represent the means ± S.E.M. of 4-5 separate experiments; *p<0.05, **p<0.01 versus control by ANOVA and a Student-Newmann-Keuls post-hoc test. (B) Bovine adrenal glomerulosa cells were incubated for 1 hour with a bicarbonate-buffered Kreb’s Ringer solution with 2.5 mM sodium acetate (KRB+) containing no additions (Con) or 100 nM PMA in the presence or absence of 2 μM thapsigargin (Thaps) or vehicle (0.1-0.2% DMSO). Supernatants were collected and assayed for aldosterone secretion by radioimmunoassay. Values represent the means ± S.E.M. of 4-5 separate experiments, expressed as the percentage of the maximal aldosterone secretory response; *p<0.05, **p<0.01 versus control by ANOVA and a Student-Newmann-Keuls post-hoc test.