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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: J Endocrinol. 2009 Apr 16;202(1):77–86. doi: 10.1677/JOE-09-0119

Figure 6.

Figure 6

Inhibition of Store-operated Ca2+ Influx with BTP2 (YM-58483) Had No Effect on AngII-induced, and Increased Elevated [K+]e-elicited PLD Activation. (A) [3H]Oleate-prelabeled cells were incubated for 30 minutes with KRB+ containing 0.5% ethanol and vehicle (0.1% DMSO) or 10 nM AngII in the presence or absence of 1 μM BTP2 for 30 minutes. Reactions were terminated by the addition of 0.2% SDS containing 5 mM EDTA, and [3H]phosphatidylethanol was extracted, separated by thin-layer chromatography and quantified as in (Jung et al., 1998). Values are expressed as -fold over the control and represent the means (± SEM) of 4 separate experiments; *p<0.05 versus the control value. (B) [3H]Oleate-prelabeled cells were incubated for 30 minutes with 3.5 mM K+-KRB+ or 15 mM K+-KRB+ containing 0.5% ethanol with vehicle (0.1% DMSO) or 1 μM BTP2 for 30 minutes, and PLD activity monitored as above. Values are expressed as -fold over the control and represent the means (± S.E.M.) of 4 separate experiments; *p<0.05 versus the control value.