Figure 7.

Effects of Eltd1 Silencing on Tumor Growth and Angiogenesis in an Orthotopic Ovarian Cancer Model

(A and B) Eltd1 siRNA/CH-NP treatment reduces tumor weight (A) and nodule number (B) in a SKOV3ip1 orthotopic mouse model.

(C) Reduced tumor growth was associated with a strong improvement in mice survival.

(D–H) Analysis of tumor tissue sections shows that Eltd1 silencing reduces MVD (D; CD31 IHC) and tumor tissue proliferation (E; Ki67 IHC) but also increases hypoxic areas (F; CA9 IHC), EC apoptosis (G; CD31/TUNEL immunofluorescence [IF]), and vascular pericyte coverage (H; CD31/desmin IF). Representative pictures are shown. Arrows indicate blood vessels within insets. Scale bars: 50 μm.

(I) Drastic reduction in metastatic spread upon Eltd1 silencing was also observed. All graph bars represent average ±SEM (unpaired t test).

See also Figure S7.