Figure 4. Monitoring H3K9ac in living organisms.

(a) Localization of H3K9ac-mintbody v1 on polytene chromosomes in salivary gland. Left and middle; bright-field and EGFP images of the whole salivary gland, respectively. Scale bar, 100 μm. Right; magnified image of the nucleus boxed in the middle panel. Scale bar, 10 μm. (b) Localization of H3K9ac-mintbody v1 with respect to Hoechst33342 staining in fixed salivary gland. Individual and merged confocal images are shown with magnified views (1,2). Heterochromatin is depleted in H3K9ac (red arrows). Some bands are particularly depleted or enriched in H3K9ac (red or green arrowheads). Scale bar, 10 μm. (c) H3K9ac-mintbody v1 in zebrafish. Boxed area is magnified in inset. Scale bar, 50 μm.