(A) Western blot (full-length) of serum probed with the follistatin antibody (upper panel). Recombinant FST288, used as the control, is detected in the blot and labeled as R-FST. Bands of serum albumin, assessed as a loading control, are presented in the lower panel. (B) The densitometry of the average western blot signals from each group as obtained in A. The fold change with respect to the FST 0 group is presented above the bar. (C) Western blot (full-length) of serum from three animals of three groups, as indicated, probed with the anti-His antibody. * and *** indicate p < 0.05 and <0.001, respectively.