Figure 3.
(A) Heteromerization of long and short forms of PIST. (A) GFP antibody was used to precipitate CFP-tagged PIST isoforms out of lysates of transfected HEK293 cells. PIST isoforms were detected using a polyclonal antibody against PIST. Bands compatible with both the GFP-tagged forms and with endogenous PIST were detected, indicating association between the transfected CFP-PIST and the endogenous one. (B) Co-precipitation of PIST isoforms with KV10.1_BBS. Lysates of KV10.1_BBS-expressing cells were labeled with an α-BTX-biotin conjugate and pulled down with streptavidin-coated beads (see Figure 1). Anti-PIST specific antibodies detected co-immunoprecipitation of bands compatible with the corresponding CFP-tagged form, together with an endogenous PIST band.