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Over-expression of PIST isoforms differently influences cellular fractions of KV10.1_BBS. Different labeling protocols allowed distinguishing between whole cell, membrane bound and internalized KV10.1_BBS by ELISA (see Materials and Methods). The fraction of membrane bound KV10.1_BBS, labeled on living cells, was clearly enriched by expression of PIST but not of the other isoforms (N = 3; ***p < 0.001).
