Table 2.
Deracemization of the sulfate esters rac-1 a–7 a using retaining PAS and inverting PISA1.
| Substrate | Reaction Type | tPISA1 [h] | tPAS [h] | Conv. [%] | eeP [%] |
|---|---|---|---|---|---|
| rac-1 a | C | 24 | 24 | 93 | 93 (S) |
| rac-1 a[a] | C | 24 | 24 | 98 | 95 (S) |
| rac-1 a[a,b] | C | 24 | 24 | 93 | 98 (S) |
| rac-2 a | A | 12 | 36 | 81 | 91 (S) |
| rac-3 a | A | 6 | 18 | >99 | 97 (S) |
| rac-4 a | B | 72 | 24 | >99 | 94 (R) |
| rac-5 a | B | 48 | 24 | >99 | >99 (R) |
| rac-6 a | B | 48 | 24 | 97 | >99 (R) |
| rac-7 a | C | 72 | 72 | 80 | >99 (S) |
See the Supporting Information for reaction conditions. [a] Double enzyme concentrations. [b] Cosolvent 20 % (v/v) DMSO.