Figure 7.

The rate of ATP hydrolysis initiated by uncoupling was measured as the rate of decline in the medium pH upon addition of MitoDNP-SUM or DNP. Experiments were performed in a Seahorse XF24 and calibrated by the addition of a known amount of acid (see the Experimental Section). Results represent means±S.E. The increased rate of ATP hydrolysis under high-ROS conditions was statistically significant under the 20 and 40 μm test conditions (p<0.05), as determined with a Student's unpaired t-test.