Fig. 3.

Specific repression of APOBEC3G expression by HIV-1 or SIVagm Vif. 293T cells were transfected as described in Fig. 1A. At the same time that the virus containing supernatant was harvested for analysis, the transfected cell cultures were lysed and the level of expression of the indicated APOBEC3G proteins was determined by Western blot analysis by using a mouse mAb specific for the HA epitope tag. A parallel Western blot analysis measuring Gag protein expression is shown in Fig. 7.