Fig. 3.

Ost4p secondary structure as determined by solution NMR spectroscopy. (a) Temperature coefficient for amide protons. Filled circles indicate coefficients more positive than -4.5 ppb/K (hydrogen bond present), and open circles indicate coefficients more negative than -4.5 ppb/K. (b) Summary of sequential and medium-range NOE connectivities. (c) Deviations of Hα chemical shifts from random coil values. (d) PsiCSI prediction of secondary structure. Negative bars correspond to helical conformation. (e) Secondary structure of Ost4p as observed in the 3D structure.