Figure 5. Equivalent NP antibody responses by wildtype B6 and B6^lpr/+ mice.

Mice were immunized with 100 μg of NP-chicken gamma globulin (CGG) in alum i.p. at day 0. Sera from indicated time points were tested for IgG anti-(NP-BSA). Relative affinity was calculated using the ratio of binding to NP₄-BSA versus NP₃₀-BSA. Values represented by each point were obtained from binding curves generated with serial 2-fold dilutions of sera. Secondary injection was performed i.p. on day 22 with 100 μg of NP-CGG in PBS and is indicated in graph by a red arrow. Day 7b refers to sera obtained 7 days after the secondary injection. Total NP Abs were calculated using the B1–8 mAb as a standard. (A) Impaired affinity maturation in B6^lpr/lpr compared to B6 mouse, immunized with NP-CGG. (B–C) Normal kinetics, quantities and affinity maturation in serum from B6^lpr/+ immunized with NP-CGG.