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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Cancer Res. 2012 Aug 20;72(20):5396–5406. doi: 10.1158/0008-5472.CAN-12-0474

Figure 2.

Figure 2

GD promotes expression of proangiogenic mediators in tumor cells. A, UM-SCC-81B cells were treated with glucose gradient (2 – 25 mM) for 18 hours. PERK, XBP1s, ATF4 and Grp78 were used as indicators of UPR activation. VEGF secretion was quantified with ELISA. B, expression of IL6, VEGF, FGF2, CTGF and Grp78 in response to GD (0.55 mM and 2 mM) was assessed with RT-PCR, and 18s was used as an internal control. C, expression of UPR markers and angiogenic factors in UM-SCC-81B cells treated with GD (2 mM, 24 hours) were determined using q-PCR. Cytokine secretion was evaluated with ELISA. D, UM-SCC-81B cells were treated with GD (2 mM), TM (1 µg/mL) and TG (1 µM). CXCL10 expression was quantified with q-PCR and normalized to control (percentage). CXCL10 secretion was quantified with ELISA. *: p < 0.05.