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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Cancer Res. 2012 Aug 20;72(20):5396–5406. doi: 10.1158/0008-5472.CAN-12-0474

Figure 4.

Figure 4

HIF1α is not involved in production of UPR-mediated proangiogenic mediators. Stable cell lines 81B-scshRNA, 81B-shHIF1α1 and 81B-shHIF1α2 were established with lentiviral vectors and treated with GD (2 mM) or CoCl2 for 24 hours. Cells cultured in regular glucose (25 mM) were used as untreated control (NT). A, UPR markers and HIF1α were assessed with western blot. Grp78 expression was determined by q-PCR. B, ELISA shows VEGF levels in the supernatant. C, expression of angiogenic factors was quantified with q-PCR and normalized to untreated control. *: p < 0.05.