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. Author manuscript; available in PMC: 2014 Jul 5.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jun 7;436(3):462–466. doi: 10.1016/j.bbrc.2013.05.127

Fig. 2. UFP increased protein S-glutathionylation in HAEC.

Fig. 2

(A) HAEC were treated with or without 50μg/mL of UFP for 6 hours, cellular levels of GSH and GSSG were measured as described in Methods. UFP decreased GSH level, but increased GSSG level. (n=4) (B) HAEC were treated with or without 50μg/mL of UFP for 6 hours and protein lysates were collected. Western blots with antibody against GSH revealed an increase in a dominant band to S-Glutathionylated Actin. The western blot with anti-Tubulin was performed as loading reference. (C) Sections of endocardium from LDLR-null mice exposed to filtered air (FA) or UFP for 10 weeks were stained with anti-GSH antibody for visualization of protein S-glutathionylation. UFP exposure led to a prominent staining in the endothelium of endocardium (Arrow).