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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: Vaccine. 2012 Jul 24;30(40):5830–5838. doi: 10.1016/j.vaccine.2012.07.033

Figure 3.

Figure 3

Expansion of CD4+/CD8+ double-positive memory cells following challenge in pigs vaccinated with LAIV. Peripheral blood mononuclear cells were collected from pigs following prime-boost vaccination with live-attenuated influenza virus (LAIV), whole-inactivated virus (WIV), or cell-culture media (NV) 5 days following pH1N1 intratracheal challenge (Ch) or non-challenge controls (NC). PBMCs were labeled with PKH67 and restimulated for five days in vitro with UV-Ca/04 or mock media and the A) percent proliferation and the percent of B) CD4+CD8− C) CD4−CD8+ and D) CD4+/CD8+ double positive cells determined using flow cytometry. Phenotypic data is expressed as the percentage of cells detected in wells following UV-Ca/04 exposure minus wells given media alone. For proliferation, a student’s t-test was used for statistical analysis of UV-Ca/04 stimulated compared to media alone and for phenotypic data a one-way analysis of variance with a Tukey’s post-test was used and p-values <0.05 are indicated with a connecting bar or asterisk.