Fig. 1.

Injection of SAHA inhibits in vivo proinflammatory cytokine production after BMT. B6D2F1 mice were given 1,300 cGy of total body irradiation and received transplants of 5 × 10⁶ T cell-depleted BM cells and 2 × 10⁶ T cells from either allogeneic (B6) or syngeneic (B6D2F1) donors as in Materials and Methods. Each F₁ recipient of the allogeneic cells were injected i.p. with 35 mg·kg^-1·day^-1 SAHA or the diluent control from day +3 to day +7 after transplantation. Sera from the recipient animals (n = 3 per group) were obtained by retroorbital venous puncture on day 7 after BMT and analyzed, as described in Materials and Methods. (a) Serum TNF-α levels are reduced in the recipients treated with SAHA. Shown are syngeneic (□) and allogeneic controls (▪) vs. SAHA allogeneic (allo) recipients (); ^*, P < 0.05. Results from one of two similar experiments are shown. (b) Serum IL1-β levels are reduced. Shown are syngeneic (□), allo controls (▪) vs. SAHA-treated allo recipients (); ^**, P < 0.02. Data are from one of two similar experiments. (c) Serum IFN-γ levels are reduced. Shown are syngeneic (□), allo controls (▪) vs. SAHA-treated allo recipients (); ^**, P < 0.02. Data from one of three similar experiments are shown.