Fig. 1.

Analysis of human:AGM APOBEC3G chimeras maps the Vif specificity-determining region to residues 1-143. (A) Structure of human:AGM APOBEC3G chimeras. Filled bars denote human sequence; open bars denote AGM sequence. The Zn²⁺-coordination domains are boxed. (B) Luciferase reporter virus assay of chimeric APOBEC3Gs. Wild-type and Δvif luciferase reporter viruses were produced in 293T cells cotransfected with chimeric APOBEC3G expression. vector, Empty vector control. Reporter virus infectivity was measured on HOS.CD4 cells infected with viruses normalized to 1.0 ng of p24. The data are the average of triplicates ± SD. Chimeric APOBEC3G expression in the transfected 293T producer cells detected on immunoblots is shown in Lower.