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. 2013 Jul 7;288(32):22915–22929. doi: 10.1074/jbc.M113.484337

FIGURE 2.

FIGURE 2.

A, representative images of dystrophin expression (red) in control and post-ischemic muscle at 3 and 14 days. Nuclei were stained with TOPRO3 (red). Magnification, ×40. B and C, analysis of nNOS expression in control and ischemic muscles by confocal microscopy (B; magnification, ×63) and Western blotting (C). Nuclei were stained with TOPRO3 (blue). The graph shows the relative level of nNOS normalized to loading control (GAPDH). *, p < 0.05 versus control. D, evaluation of iNOS expression in control and ischemic muscles at 3, 7, 14, and 21 days by Western blotting and confocal microscopy. E and F, evaluation of eNOS and nNOS (E) and dystrophin (F) in ischemic muscles from control (vehicle) and MC1568- or MS275-treated mice at 3 and 14 days after ischemia. Error bars, S.E. AU, arbitrary units.